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Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI
Author(s) -
Naitou Masanori,
Ozawa Masashi,
Sasanuma SyunIchi,
Kobayashi Motoe,
Hagiwara Hiroko,
Shibata Takehiko,
Hanaoka Fumio,
Watanabe Koji,
Ono Ayako,
Yamazaki Masaaki,
Tashiro Hiroyuki,
Eki Toshihiko,
Murakami Yasufumi
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199601)12:1<77::aid-yea887>3.0.co;2-5
Subject(s) - orfs , biology , clone (java method) , genetics , genbank , open reading frame , saccharomyces cerevisiae , accession number (library science) , plasmid , gene , nucleic acid sequence , transposable element , chromosome , genome , whole genome sequencing , microbiology and biotechnology , peptide sequence
Plasmid clone gapB and lambda phage clone 4682, which contain fragments of Saccharomyces cerevisiae chromosome VI, were analysed. A 23 kb sequence was determined and ten open reading frames (ORFs) were revealed. Among them, five ORFs were identical to five yeast genes ( SEC4, MSH4, SPB4, DEG1 and NIC96 ), two were identical to transposable elements ( TYA and TYB ), one (gapBorfF003) was highly homologous to a yeast expressed sequence tag, and another (4682orfF002) was predicted to be a nuclear protein. Sequence data have been submitted to DDBJ/EMBL/GenBank data library under Accession Number D44604 (clone gapB) and D44600 (clone 4682), respectively.