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Effects of androgens on telomerase activity in normal and malignant prostate cells in vitro
Author(s) -
Soda Hiroshi,
Raymond Eric,
Sharma Sunil,
Lawrence Richard,
Davidson Karen,
Oka Mikio,
Kohno Shigeru,
Izbicka Elzbieta,
Von Hoff Daniel D.
Publication year - 2000
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/(sici)1097-0045(20000515)43:3<161::aid-pros1>3.0.co;2-o
Subject(s) - telomerase , prostate , in vitro , medicine , prostate disease , endocrinology , prostate cancer , cancer research , oncology , biology , cancer , genetics , gene
BACKGROUND Recent studies have shown that sex hormones regulate telomerase activity in endometrium and breast tissues. The present study was designed to clarify the effects of androgen on telomerase activity in normal and malignant prostate cells. METHODS Androgen‐sensitive (LNCaP) and ‐independent (TSU‐Pr1 and DU145) prostate cancer cell lines and normal prostate cells including basal cells were cultured in the presence or absence of 5α‐dihydrotestosterone (DHT). RESULTS Prostate cancer cell lines exhibited high telomerase activity, and normal prostate cells showed low activity. Short or prolonged androgen‐deprivation reduced telomerase activity in LNCaP cells, and DHT induced telomerase activity at the G 1 phase of the cell cycle. DHT did not modulate telomerase activity in TSU‐Pr1, DU145, and normal cells. CONCLUSIONS LNCaP cells had an androgen‐dependent pathway to activate telomerase, whereas TSU‐Pr1 and DU145 cells as well as normal prostate cells had an androgen‐independent pathway. These findings suggest that the regulatory mechanism of telomerase varies during the progression of prostate cancers. Prostate 43:161–168, 2000. © 2000 Wiley‐Liss, Inc.