Premium
High‐malignancy orthotopic nude mouse model of human prostate cancer LNCaP
Author(s) -
Wang Xiaoen,
An Zili,
Geller Jack,
Hoffman Robert M.
Publication year - 1999
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/(sici)1097-0045(19990515)39:3<182::aid-pros6>3.0.co;2-b
Subject(s) - lncap , medicine , prostate cancer , metastasis , prostate , malignancy , pathology , cancer , primary tumor , micrometastasis , lymph node , cancer research
BACKGROUND An animal model of human prostate cancer LNCaP demonstrating high rates of spontaneous metastasis from the orthotopic site after tumor implantation would be very valuable for mechanistic and drug discovery studies. We previously developed microsurgical techniques to implant histologically intact tumor tissues orthotopically in nude mice in order to develop high metastatic mouse models of human cancer. METHODS Intact tissue of the androgen‐dependent human prostate cancer cell line, LNCaP, was implanted on the ventral lateral lobes of the prostate gland by surgical orthotopic implantation (SOI) in a series of 20 nude mice. Mice were autopsied, and histopathological examination of primary tumors and relevant organs was done to identify and quantitate micrometastasis. RESULTS Eighteen of 20 animals transplanted with LNCaP by SOI had tumor growth. Mean primary tumor weight in the prostate was 9.24 g at time of necropsy. Sixty‐one percent of the transplanted animals had lymph node metastasis. Forty‐four percent had lung metastasis. Mean survival time was 72 days, indicating a high degree of malignancy of the tumor. CONCLUSIONS The extensive and widespread lung metastasis as well as lymph node metastasis following orthotopic implantation of LNCaP in nude mice and the short survival time provide a high‐malignancy nude model of the LNCaP human prostate tumor. Prostate 39:182–186, 1999. © 1999 Wiley‐Liss, Inc.