Premium
Establishment of prostatic cell line “Pro9ad” from a p53‐deficient mouse
Author(s) -
Hanazono Makoto,
Nakagawa Eriko,
Aizawa Shinichi,
Tomooka Yasuhiro
Publication year - 1998
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/(sici)1097-0045(19980701)36:2<102::aid-pros5>3.0.co;2-k
Subject(s) - endocrinology , epidermal growth factor , keratinocyte growth factor , medicine , biology , growth factor , hepatocyte growth factor , fibroblast growth factor , cell culture , prostate , epithelium , cell growth , immortalised cell line , dihydrotestosterone , cancer research , androgen , receptor , hormone , cancer , genetics
BACKGROUND We demonstrated that p53‐deficiency is sufficient for immortalization of fetal uterine cells. In the present study, we further extended our previous observations to prostate tissues from a young p53‐deficient adult mouse. METHODS Cell lines were established from the ventral prostate of a p53‐deficient male mouse and maintained in medium containing 10% heat‐inactivated fetal calf serum supplemented with insulin (10 μg/ml), transferrin (10 μg/ml), cholera toxin (10 ng/ml), and selenium (10 −8 M). RESULTS Pro9ad, one of the lines established, exhibits a typical epithelial morphology in culture. Despite the possession of androgen receptors, the growth of Pro9ad was not stimulated by 5α‐dihydrotestosterone. Hepatocyte growth factor (HGF) slightly stimulated proliferation, whereas fibroblast growth factor‐1 (FGF‐1), keratinocyte growth factor (KGF), and platelet‐derived growth factor AB (PDGF‐AB) had no stimulating effect on growth. However, FGF‐2, epidermal growth factor (EGF), and insulin‐like growth factor‐1 (IGF‐1) accelerated proliferation in a dose‐dependent manner. EGF and IGF‐1 additively stimulated growth. CONCLUSIONS These results suggest that Pro9ad shares characteristics in common with primary prostatic epithelial cells despite p53‐deficiency, and that p53‐deficiency alone allows establishment of clonal cell lines of the prostate epithelium. Furthermore, the prostates of p53‐deficient mice are useful sources for obtaining cell lines. Prostate 36:102–109, 1998. © 1998 Wiley‐Liss, Inc.