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Development and characterization of a mouse prostate adenocarcinoma cell line: Ductal formation determined by extracellular matrix
Author(s) -
Jorcyk Cheryl L.,
Liu MinLing,
Shibata MasaAki,
Maroulakou Ioanna G.,
Komschlies Kristin L.,
McPhaul Michael J.,
Resau James H.,
Green Jeffrey E.
Publication year - 1998
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/(sici)1097-0045(19980101)34:1<10::aid-pros2>3.0.co;2-o
Subject(s) - extracellular matrix , prostate , adenocarcinoma , prostate cancer , pathology , prostate disease , cell culture , medicine , biology , cancer research , microbiology and biotechnology , cancer , genetics
BACKGROUND Tumor vaccines show promise as a new approach for treating cancer. We have developed a murine prostate cancer cell line which can be used to study growth factor and extracellular matrix regulation of prostate differentiation and will be useful for generating tumor vaccines using the C3(1)/T AG transgenic model of prostate cancer. METHODS Pr‐14 cells were established in defined growth media (GM) and grown in GM, GM + 2% fetal bovine serum (FBS) or DMEM + 10% FBS on plastic, collagen, or Matrigel. Immunofluorescence and Western blot analyses were performed using antibodies to cytokeratin, vimentin, SV40 large T‐antigen, and androgen receptor (AR). RESULTS Pr‐14 cells are cytokeratin‐positive, vimentin‐negative, and express SV40 large T‐antigen. These cells are tumorigenic when injected into athymic nude mice and appear to be androgen‐independent. Pr‐14 cell lines are nontumorigenic when injected into syngeneic FVB/N mice, but form tumors in transgenic T AG ‐expressing FVB/N mice. Cell growth and morphology are dependent on media composition which determines whether ductal or acinar structures form when grown on Matrigel. CONCLUSIONS The mouse prostate adenocarcinoma cell line, Pr‐14, undergoes alterations in the state of differentiation dependent upon serum concentration when grown on Matrigel. The Pr‐14 cell line is a useful reagent to study prostate cell/extracellular matrix interactions, and for immunotherapy and cancer vaccine studies in C3(1)/T AG transgenic mice. Prostate 34:10–22, 1998. © 1998 Wiley‐Liss, Inc.