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HHV‐8 (KSHV) does not establish latency in prostate cancer cell lines
Author(s) -
Munker Reinhold,
Tasaka Taizo,
Park Dorothy,
Miller Carl W.,
Koeffler H. Phillip
Publication year - 1997
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/(sici)1097-0045(19971201)33:4<286::aid-pros10>3.0.co;2-f
Subject(s) - prostate cancer , prostate , medicine , biology , cancer research , cancer , oncology , pathology
BACKGROUND HHV‐8 is a new herpesvirus found in lesions of Kaposi's sarcoma and some lymphoproliferative diseases. More recently, a report stated that normal prostate tissue also contains the virus. METHODS The expression of HHV‐8 was examined by a sensitive reverse‐transcriptase PCR for the viral genes ORF 72, ORF 73, ORF 74, and ORF 75. In coculture experiments we attempted to infect 3 commonly studied prostate cancer cell lines using induced and uninduced lymphoid cell lines harboring HHV‐8 (KS‐1, BC‐1, and BC‐2). For induction of viral genes, butyrate and phorbol esters were used. RESULTS AND CONCLUSIONS. At baseline, prostate cancer cell lines LNCaP, DU‐145, and PC‐3 did not express viral gene products. Extensive coculture experiments were also negative. In no instance could latency for the virus be established. Our results argue against the involvement of HHV‐8 in prostate cancer and for a limited tissue tropism of HHV‐8. Prostate 33:286–288, 1997. © 1997 Wiley‐Liss, Inc.