Premium
Orthotopic implantation of human prostate cancer cell lines: A clinically relevant animal model for metastatic prostate cancer
Author(s) -
Rembrink Klaus,
Romijn Johannes C.,
van der Kwast Theo H.,
Rübben Herbert,
Schröder Fritz H.
Publication year - 1997
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/(sici)1097-0045(19970515)31:3<168::aid-pros4>3.0.co;2-h
Subject(s) - prostate , prostate cancer , medicine , cancer , pathology , oncology , metastasis , cancer research
BACKGROUND To study the metastatic behavior of human prostate cancer cell lines, orthotopic injection in nude mice was performed. METHODS Local tumor growth, metastasis formation, prostate‐specific antigen, and androgen receptor expression were examined. RESULTS Hormone‐independent cell lines (PC‐3, PC‐3‐125‐1L, and TSU‐Pr1) were highly tumorigenic and had a higher rate of lymph node metastasis after orthotopic than after subcutaneous implantation. PC‐3 cell lines also consistently metastasized to the lungs. The androgen‐sensitive LNCaP cell line showed local growth in 7 of 10, and lymph node metastasis in 4 animals. Significant serum PSA levels and strong receptor expression in primary and metastatic tumor tissues were observed. CONCLUSIONS These results demonstrates that orthotopic implantation of human prostate cancer cell lines, including LNCaP, reproducibly leads to metastasis formation in nude mice. Prostate 31:168–174, 1997. © 1997 Wiley‐Liss, Inc.