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Effect of retinoic acid on prostatic development
Author(s) -
Aboseif Sherif R.,
Dahiya Rajvir,
Narayan Perinchery,
Cunha Gerald R.
Publication year - 1997
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/(sici)1097-0045(19970515)31:3<161::aid-pros3>3.0.co;2-o
Subject(s) - retinoic acid , retinoid , prostate , retinoic acid receptor , endocrinology , receptor , medicine , morphogenesis , tretinoin , biology , cancer research , andrology , gene , biochemistry , cancer
BACKGROUND AND METHODS. To assess the effect of retinoids on prostatic ductal branching morphogenesis, anterior prostates from newborn rats were cultured under serum‐free conditions for 6 days in the presence of testosterone (10 −8 mM) plus 13‐cis‐retinoic acid (13‐cis‐RA), all‐trans‐retinoic acid (at‐RA), or N‐4‐hydroxyphenyl‐retinamide (4‐HPR). Measures of morphologic complexity were computed and compared between specimens of different treatment groups. RESULTS Prostatic ductal growth and branching were inhibited in a dose‐dependent fashion by both 13‐cis‐RA and at‐RA, but not by 4‐HPR. This inhibitory effect of 13‐cis‐RA was reversible, as the prostatic ducts resumed branching and growth after removal of retinoic acid from the culture medium. Using reverse transcription polymerase chain reaction, we then investigated the expression of nuclear receptor genes for retinoic acid. CONCLUSIONS This showed the presence of RAR‐β and RAR‐γ in the 0‐day prostate, suggesting that the effects of these retinoids on ductal morphogenesis may be via these receptors. Prostate 31:161–167, 1997. © 1997 Wiley‐Liss, Inc.