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Characterization of intracellular calcium mobilization by bombesin‐related neuropeptides in PC‐3 human prostate cancer cells
Author(s) -
Han Kehan,
Viallet Jean,
Chevalier Simone,
Zheng Wei,
Bazinet Michel,
Aprikian Armen G.
Publication year - 1997
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/(sici)1097-0045(19970401)31:1<53::aid-pros9>3.0.co;2-j
Subject(s) - bombesin , neuropeptide , endocrinology , stimulation , medicine , calcium in biology , intracellular , calcium , chemistry , biology , receptor , microbiology and biotechnology
BACKGROUND The objective of this study was to characterize the calcium mobilizing activity of bombesin‐related neuropeptides in PC‐3 cells. METHODS Intracellular free Ca 2+ concentration ([Ca 2+ ] i ) was measured following the administration of bombesin‐related neuropeptides using spectrofluorometric monitoring of PC‐3 cells loaded with Fura2/AM. RESULTS Individual neuropeptides induced a rapid rise in [Ca 2+ ] i immediately after stimulation in similar fashion. When two different peptides were administered simultaneously, the [Ca 2+ ] i changes were similar to that produced by each peptide alone. However, simultaneous addition of tyr 4 ‐bombesin 100 nM and 2% serum resulted in [Ca 2+ ] i increase greater than either alone. Sequential stimulation by same or different peptides, was not able to induce another response. However, 6 hrs after the first stimulus, a second stimulus was able to induce a similar response. CONCLUSIONS The data indicate that all members of the bombesin‐related family of neuropeptides are capable of triggering calcium mobilization in PC‐3 cells via a similar signalling pathway. PC‐3 cells become acutely desensitized to additional stimulation of neuropeptide. This desensitization is selective and reversible, requiring approximately 6 hrs for recovery. Prostate 31:53–60, 1997. © 1997 Wiley‐Liss, Inc.