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Effects of the lipidosterolic extract of Serenoa repens (Permixon®) on human prostatic cell lines
Author(s) -
Ravenna L.,
Di Silverio F.,
Russo M. A.,
Salvatori L.,
Morgante E.,
Morrone S.,
Cardillo M. R.,
Russo A.,
Frati L.,
Gulino A.,
Petrangeli E.
Publication year - 1996
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/(sici)1097-0045(199610)29:4<219::aid-pros3>3.0.co;2-6
Subject(s) - lncap , androgen receptor , endocrinology , androgen , medicine , hyperplasia , receptor , prostate cancer , prostate , cancer research , biology , cancer , hormone
BACKGROUND Permixon® is a drug used in the treatment of benign prostatic hyperplasia. We studied its androgenic and antiandrogenic effects in the prostatic cell lines LNCaP and PC3, respectively responsive and unresponsive to androgen stimulation. METHODS We performed FACScan analysis to investigate toxicity, 3 H thymidine and 35 S methionine incorporation to determine antiproliferative and metabolic effects, electron microscopy to study ultrastructural changes and cotransfection experiments to elucidate the role of wild type androgen receptor. RESULTS In LNCaP cell line, Permixon® induced a double proliferative/differentiative effect, not observed in PC3 cells. In PC3 cells cotransfected with wild‐type androgen receptors and CAT reporter genes under the control of a androgen responsive element, the drug inhibited androgen‐induced CAT transcription. CONCLUSIONS Our data indicate a role of the androgen receptor in mediating the effects of Permixon® in LNCaP cells. Cotransfection experiments in PC3 cells support a clear antiandrogenic action of the drug. © 1996 Wiley‐Liss, Inc.