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Evaluation of fixation methods for ultrastructural study of Caenorhabditis elegans embryos
Author(s) -
Vancoppenolle Bart,
Claeys Myriam,
Borgonie Gaetan,
Tytgat Tom,
Coomans August
Publication year - 2000
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/(sici)1097-0029(20000415)49:2<212::aid-jemt15>3.0.co;2-1
Subject(s) - ultrastructure , biology , embryo , caenorhabditis elegans , fixation (population genetics) , microbiology and biotechnology , embryogenesis , anatomy , genetics , gene
Various fixation methods for transmission electron microscopy (TEM) were tested on Caenorhabditis elegans embryos. By combining various techniques, using 3.4% chitinase in combination with 1% α‐chymotripsin, we were able to establish a new fixation procedure that for the first time preserves both membranes and internal cellular ultrastructure of C . elegans embryos in different stages of development. This unique procedure will enable a hitherto unattainable standard for TEM research of C . elegans embryos. Sectioning of specific developmental stages fixed with this method allows a detailed study of ultrastructural aspects of embryogenesis. Microsc. Res. Tech. 49:212–216, 2000. © 2000 Wiley‐Liss, Inc.