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Role of mediatophore in connection with proteins of the active zone in synaptic transmission
Author(s) -
Morel N.,
Israël M.
Publication year - 2000
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/(sici)1097-0029(20000401)49:1<47::aid-jemt6>3.0.co;2-w
Subject(s) - neurotransmission , active zone , depolarization , biophysics , calcium , acetylcholine , chemistry , microbiology and biotechnology , cytosol , membrane , biology , biochemistry , synaptic vesicle , enzyme , receptor , vesicle , organic chemistry , endocrinology
Mediatophore is a protein purified from Torpedo electric organ synaptosomes, which translocates acetylcholine (ACh) upon calcium action after reconstitution in artificial membranes. After expression in transfected cells, it endows these cells with a calcium‐dependent release mechanism displaying clear quantal properties. The role of mediatophore in synaptic transmission is discussed in relation to the ultrastructural organization of the active zone and the cytosolic high calcium microdomains that transiently appear after presynaptic membrane depolarization. Microsc. Res. Tech. 49:47–55, 2000. © 2000 Wiley‐Liss, Inc.