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Fluorescence microscopy of etched methacrylate sections improves the study of mitosis in plant cells
Author(s) -
Hoffman John C.,
Vaughn Kevin C.,
Mullins J. Michael
Publication year - 1998
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/(sici)1097-0029(19980301)40:5<369::aid-jemt4>3.0.co;2-m
Subject(s) - cytoskeleton , confocal microscopy , mitosis , fluorescence microscope , confocal , immunofluorescence , microtubule , biophysics , microscopy , methacrylate , plant cell , microbiology and biotechnology , fluorescence , polymerization , chemistry , biology , cell , pathology , biochemistry , antibody , optics , polymer , medicine , physics , organic chemistry , gene , immunology
Etched sections of methacrylate infiltrated plant tissue [Gubler (1989) Cell Biol. Int; Rep., 13:137–145; Baskin et al. (1992) Planta, 187:405–413] offer many advantages over the more traditional squash technique of Wick et al. [(1981) J. Cell Biol. 89:685–690] for immunofluorescence microscopic investigation of the plant cytoskeleton, especially during mitosis. These advantages include: (1) unimpeded access of antibody probes, (2) confocal‐like imaging without the expense of confocal equipment, (3) maintenance of organ architecture as well as intracellular structure, (4) the ability to independently examine separate focal planes with the same or multiple antibody(s) or other labelling compounds, and (5) the ability to archive unetched sections, polymerized or non‐polymerized infiltrated tissue. In this paper examples of staining of various microtubule cytoskeletal and mitotic proteins are shown in a variety of methacrylate embedded plant tissues. Microsc. Res. Tech. 40:369–376, 1998. © 1998 Wiley‐Liss, Inc.