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Three‐dimensional cultures of keratinocytes and an application to in vitro‐amyloid production of cutaneous amyloidosis
Author(s) -
Horiguchi Yuji,
Suzuki Shigehiko,
Toda KenIchi,
Ueda Masamichi,
Leigh Irene M.,
Imamura Sadao
Publication year - 1997
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/(sici)1097-0029(19970815)38:4<387::aid-jemt6>3.0.co;2-g
Subject(s) - hemidesmosome , lamina densa , keratinocyte , basement membrane , basal lamina , extracellular matrix , microbiology and biotechnology , epidermis (zoology) , dermoepidermal junction , pathology , lamina lucida , amyloid (mycology) , chemistry , anchoring fibrils , biology , in vitro , laminin , anatomy , dermis , medicine , biochemistry , ultrastructure
Some three‐dimensional culture models of the skin were reviewed including our systems using a collagen dermal substitute and a matrix dermal substitute. No obvious junctional structures, such as hemidesmosomes and the lamina densa, were formed between the basal keratinocytes and the dermal substitutes, when the cytoplasmic membrane of the basal keratinocytes directly faced the collagenous materials. On the other hand, when the cytoplasmic membrane of the basal keratinocytes faced the preformed basement membrane, the type IV collagen film, or the extracellular matrix gel, an organized interaction occurred between the basal keratinocytes and the dermal substitute through hemidesmosomes and a rudimentary lamina densa. Keratinocyte differentiation in the suprabasal layers seemed to be closely related to such a basal cell differentiation. Our preliminary examination of the experimental amyloid production by the epidermal sheet from the lesional skin of patients with primary localized cutaneous amyloidosis suggested that the basal cells in the transplanted lesional epidermis produced amyloid fibrils in our in vitro culture model. This is another use of the three‐dimensional culture models of the skin in addition to the application of the systems to wound treatment. Microsc. Res. Tech. 38:387–393, 1997. © 1997 Wiley‐Liss, Inc.