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Acquisition of high‐resolution digital images in video microscopy: Automated image mosaicking on a desktop microcomputer
Author(s) -
Ott Swidbert R.
Publication year - 1997
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/(sici)1097-0029(19970801)38:3<335::aid-jemt14>3.0.co;2-o
Subject(s) - computer vision , computer science , microcomputer , magnification , artificial intelligence , image processing , computer graphics (images) , software , frame (networking) , digital image , digital image processing , reliability (semiconductor) , enhanced data rates for gsm evolution , digital imaging , image (mathematics) , telecommunications , chip , power (physics) , physics , quantum mechanics , programming language
For the digital processing of microscopical images, mosaicking is a prerequisite if the specimen is larger than the camera field at the necessary magnification. This study investigates the possibilities and limitations of fully automated mosaicking on a desktop computer. Cross‐correlation‐based frame registration was performed with high reliability if the video frames were edge‐enhanced before matched filtering, and also in a reasonable time since the search for matches was restricted to pairs of consecutive frames. An environment for routine mosaicking was developed and implemented in a widely used desktop image‐processing program. The software developed during this study has been released to the public domain. Microsc. Res. Tech. 38:335–339, 1997. © 1997 Wiley‐Liss, Inc.