Premium
Complementary advantages of fluorescence and SIMS microscopies in the study of cellular localization of two new antitumor drugs
Author(s) -
GuerquinKern JeanLuc,
Coppey Maïté,
Carrez Danièle,
Brunet AnneChristine,
Nguyen Chi Hung,
Rivalle Christian,
Slodzian Georges,
Croisy Alain
Publication year - 1997
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/(sici)1097-0029(19970215)36:4<287::aid-jemt6>3.0.co;2-j
Subject(s) - fluorescence , fluorescence microscope , biophysics , nucleolus , chemistry , microscopy , secondary ion mass spectrometry , cell nucleus , nucleus , dna , intracellular , kinetics , mass spectrometry , cytoplasm , microbiology and biotechnology , biochemistry , biology , pathology , medicine , physics , quantum mechanics , chromatography
Low light level fluorescence microscopy studies have been carried out on MCF7‐P human mammary tumor cells to localize the intracellular distribtion of two new anticancer drugs, Pazelliptine and Intoplicine, which are currently under clinical evaluation. These two molecules are thought to act at the nuclear level, through DNA topoisomerase interactions. Because fluorescence of these compounds appears strongly quenched by intercalation in double strand DNA, secondary ion mass spectrometry (SIMS) imaging was used to check the presence of the drugs in the nuclear compartment. In spite of chemical structure similitudes, pazelliptine and intoplicine appear to be distributed in quite different ways within the cells. Incubation for 1 and 24 hours also allowed us to bring to light strong differences in the distribution kinetics. Pazelliptine quickly enters into the nucleoli but is no longer present in the nucleus after 24 hours incubation. Intoplicine was not detected by fluorescence in the nucleus, however SIMS microscopy allowed us to show its accumulation within this cellular compartment as a function of time of exposure. This study shows the complementarity of fluorescence and SIMS microscopies. Microsc. Res. Tech. 36:287–295, 1997. © 1997 Wiley‐Liss, Inc.