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Applications of laser scanning microscopy for analysis of aquatic microhabitats
Author(s) -
Ghiorse William C.,
Miller Daniel N.,
Sandoli Robert L.,
Siering Patricia L.
Publication year - 1996
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/(sici)1097-0029(199601)33:1<73::aid-jemt7>3.0.co;2-1
Subject(s) - microscopy , in situ , fluorescence microscope , scanning electron microscope , bacteria , chemistry , biology , fluorescence , materials science , optics , genetics , physics , organic chemistry , composite material
Laser scanning microscopy was used 1) to analyze Mn oxide‐encrusted biofilms and particles in marine Mn‐oxidizing enrichment cultures, 2) to optimize fluorescence in situ hybridization protocols of 16S ribosomal RNA‐targeted oligonucleotide signature probes for single bacterial cell identification in particles from a wetland, and 3) to develop a combined immunofluorescence‐microautoradiography procedure for analysis of the distribution of 14 C‐labeled organic compounds and 14 C‐mineralizing bacteria in groundwater seep sediments. The results demonstrated the wide applicability and benefits of using laser scanning microscopy for analysis of complex microbial assemblages. © 1996 Wiley‐Liss, Inc.