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Genetic differentiation between sole ( Solea solea ) and Greenland halibut ( Reinhardtius hippoglossoides ) by PCR–RFLP analysis of a 12S rRNA gene fragment
Author(s) -
Céspedes Ana,
García Teresa,
Carrera Esther,
González Isabel,
Fernández Alicia,
Asensio Luis,
Hernández Pablo E,
Martín Rosario
Publication year - 2000
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(20000101)80:1<29::aid-jsfa470>3.0.co;2-4
Subject(s) - biology , halibut , restriction enzyme , restriction fragment length polymorphism , amplicon , genetics , agarose gel electrophoresis , hindiii , polymerase chain reaction , pleuronectidae , mitochondrial dna , fishery , gene , flounder , fish <actinopterygii>
PCR–RFLP analysis was applied to the identification of two closely related flatfish species: sole ( Solea solea ) and Greenland halibut ( Reinhardtius hippoglossoides ). Amplification of DNA isolated from fish muscle samples was carried out using a set of primers flanking a region of 321 base pairs (bp) from the mitochondrial 12S rRNA gene. Restriction endonuclease analysis based on sequence data of this DNA fragment revealed the presence of polymorphic sites for Aci I and Mwo I endonucleases. The restriction profiles obtained by agarose gel electrophoresis when amplicons were cut with Aci I and Mwo I enzymes allowed the unequivocal identification of sole and Greenland halibut species. © 2000 Society of Chemical Industry