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A method for partitioning neutral detergent‐soluble carbohydrates
Author(s) -
Hall Mary B,
Hoover William H,
Jennings Jocelyn P,
Webster Tammy K Miller
Publication year - 1999
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199912)79:15<2079::aid-jsfa502>3.0.co;2-z
Subject(s) - chemistry , ethanol , starch , fractionation , carbohydrate , chromatography , water soluble , residue (chemistry) , solubility , organic chemistry , food science
A method was developed to fractionate the neutral detergent‐soluble carbohydrates (NDSC) in feedstuffs. Differential solubilities of carbohydrates in 80:20 (v/v) ethanol/water were used to partition NDSC into organic acids (OA) and mono‐ and oligosaccharides soluble in ethanol/water from starch and neutral detergent‐soluble fibre (NDSF) which are insoluble. Mono‐ and oligosaccharides (total ethanol/water‐soluble carbohydrate) were measured on the ethanol/water extract, and starch was measured on the ethanol/water‐insoluble residue. The OA and NDSF, the two most compositionally diverse NDSC fractions, were estimated by difference. The method allows partitioning of the NDSC on a nutritionally relevant basis into (1) organic acids, (2) total ethanol/water‐soluble carbohydrate, (3) starch and (4) neutral detergent‐soluble fibre. The methods involved in this fractionation are relatively simple or are commonly used. © 1999 Society of Chemical Industry