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Characterisation of cellulose‐hydrolysing enzymes from the fungus Bipolaris sorokiniana
Author(s) -
Geimba Mercedes P,
Riffel Alessandro,
Agostini Viviane,
Brandelli Adriano
Publication year - 1999
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199910)79:13<1849::aid-jsfa445>3.0.co;2-4
Subject(s) - cellulase , cellulose , chemistry , carboxymethyl cellulose , hydrolysis , enzyme , chromatography , cellulosic ethanol , lactose , substrate (aquarium) , biochemistry , food science , organic chemistry , biology , sodium , ecology
Cellulose‐hydrolysing enzymes from the phytopathogenic fungus Bipolaris sorokiniana were partially purified and characterised. The enzyme production was variable according to the carbon source. β‐Glucosidase and cellobiohydrolase activities were higher by growing the fungus on cellulose than on other carbon sources. Carboxymethyl cellulase production was stimulated by other carbohydrates, mainly lactose. Partial enzyme purification was carried out by liquid chromatography on Sepharose CL4B. The purification was about 17‐fold, with a yield of 41% as judged by assay with p ‐nitrophenyl‐β‐ D ‐glucopyranoside as substrate. The optimum pH and temperature were 5.0 and 55–60 °C respectively. The enzyme was stable at 28 and 37 °C but lost about 50% of its initial activity after 120 min at 55 °C. Saccharification of cellulosic materials such as crystalline cellulose, filter paper and wheat straws was carried out using the partially purified enzyme, resulting in the production of reducing sugars. © 1999 Society of Chemical Industry