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Detection and quantification of goat's cheese in ewe's cheese using a monoclonal antibody and two ELISA formats
Author(s) -
Haza Ana I,
Morales Paloma,
Martín Rosario,
García Teresa,
Anguita Gonzalo,
Sanz Bernabé,
Hernández Pablo E
Publication year - 1999
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(19990515)79:7<1043::aid-jsfa326>3.0.co;2-5
Subject(s) - monoclonal antibody , food science , casein , chemistry , detection limit , cheese ripening , antibody , ripening , biology , chromatography , immunology
A stable hybridoma cell line (B2B) has been produced secreting a monoclonal antibody (MAb) specific for the s α s2 ‐casein of goats. The MAb B2B was used in two enzyme‐linked immunosorbent assays (ELISA) formats for the detection and quantification of the presence of goat's cheese in ewe's cheese samples. In the indirect ELISA format the limit of detection was 1–25% (w/w) substitution of ewe's cheese samples by goat's cheese. Afterwards, a competitive indirect ELISA was successfully developed for the detection of 0.5 to 25% (w/w) of goat's cheese in ewe's cheese samples. This competitive indirect ELISA is a very sensitive assay, can be performed in less than 5 h and is not influenced by the ripening process in cheese. © 1999 Society of Chemical Industry