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Degradation of pelargonidin 3‐glucoside in the presence of chlorogenic acid and blueberry polyphenol oxidase
Author(s) -
Kader Farid,
Nicolas JeanPierre,
Metche Maurice
Publication year - 1999
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(19990315)79:4<517::aid-jsfa212>3.0.co;2-n
Subject(s) - chemistry , chlorogenic acid , pelargonidin , polyphenol oxidase , anthocyanin , oxygen , quinone , polyphenol , pigment , high performance liquid chromatography , degradation (telecommunications) , stoichiometry , redox , organic chemistry , nuclear chemistry , chromatography , food science , cyanidin , antioxidant , enzyme , peroxidase , telecommunications , computer science
Oxidation of both chlorogenic acid (CG) and pelargonidin3‐glucoside (Pg 3‐glc) as well as theirmixture, in the presence of blueberry polyphenol oxidase(PPO) was studied in model solutions. The reactions weremonitored by spectrophotometric, polarographic and HPLC methods. Inthe absence of CG, Pg 3‐glc is not oxidised by blueberry PPO,but pigment degradation is induced by the presence of chlorogenicacid. Kinetic studies showed that Pg 3‐glc was degraded by amechanism involving a reaction between chlorogenoquinone and/orsecondary products of oxidation formed from the quinone and thepigment. Therefore, no coupled oxidation mechanisms occurred with Pg3‐glc, as expected of its structure. The oxidation of CG byPPO in the presence of Pg 3‐glc involved the consumption of0.6 μmol of oxygen per μmol of CG oxidised, which is close tothe stoichiometry calculated from the oxidation of CG alone(0.63 μmol of oxygen per μmol). The initial phase ofthe pigment degradation showed a delay which did not correspond tocresolase activity. This phase could be explained by aquinone/anthocyanin reaction which is dependent on thechlorogenoquinone concentration and/or a reaction involvingsecondary products of oxidation (formed from thechlorogenoquinone) and Pg 3‐glc. © 1999 Society of Chemical Industry