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Flavonoids quercetin, myricetin, kaemferol and (+)‐catechin as antioxidants in methyl linoleate
Author(s) -
Pekkarinen Satu S,
Hein I Marina,
Hopia Anu I
Publication year - 1999
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(19990315)79:4<499::aid-jsfa204>3.0.co;2-u
Subject(s) - myricetin , rutin , chemistry , quercetin , catechin , antioxidant , organic chemistry , polyphenol , kaempferol
The antioxidant effect of the flavonoids quercetin, myricetin,kaemferol, (+)‐catechin and rutin on methyllinoleate oxidation was investigated. In addition, the synergisticeffects of flavonoids and α‐tocopherol were studied.Oxidation was monitored by conjugated diene measurement and bydetermining the formation of hydroperoxide isomers by HPLC. Theantioxidant activity of flavonoids in non‐polar methyllinoleate differ from that previously reported inwater‐containing systems, such as LDL and liposome systems.The activity of antioxidants (10–1000 μ M )measured by hydroperoxide formation decreased in the order:myricetin>quercetin>α‐tocopherol>(+)‐catechin >kaemferol=rutin. The antioxidant activity of flavonoids increased as the number of phenolic hydroxyl groups increased. In addition to the number of hydroxyl groups, other structural features such as the 2,3 double bond in the C‐ring and a glycoside moiety in the molecule had an effect on the antioxidant activity. Myricetin and rutin, especially had a synergistic effect with α‐tocopherol. Myricetin, quercetin and rutin protected α‐tocopherol from decomposition, myricetin being the most protective. The relative hydrogen‐donating activity measured by the ratio of cis , trans ‐ to trans , trans hydroperoxide isomers formed during oxidation decreased in the order: α‐tocopherol >myricetin>quercetin. Hydroperoxide isomeric distribution of the samples containing kaemferol or rutin did not differ from the control. Thus, although α‐tocopherol was the most effective hydrogendonor, myricetin and quercetin were more effective antioxidants in inhibiting the hydroperoxide formation in methyl linoleate. © 1999 Society of Chemical Industry