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In vitro and in situ disappearance of β‐carotene and lutein from lucerne ( Medicago sativa ) hay in bovine and caprine ruminal fluids
Author(s) -
Mora Ofelia,
Romano José L,
González Elvira,
Ruiz Felipe J,
Shimada Armando
Publication year - 1999
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199902)79:2<273::aid-jsfa191>3.0.co;2-v
Subject(s) - lutein , xanthophyll , dry matter , carotene , medicago sativa , hay , carotenoid , ruminant , rumen , chemistry , saponification , food science , botany , zoology , biology , agronomy , biochemistry , pasture , fermentation
Two experiments were conducted to determine the rumen fluid disappearance rates (kd) of β‐carotene, lutein, total carotene and total xanthophyll from lucerne ( Medicago sativa ) hay, in two ruminant species: Brahman steers (fat‐pigmenting) and Granadine goats (non‐pigmenting). Within species, the in vitro and the in situ methods were compared. The concentration of carotenoid compounds was determined by spectrophotometry and high performance liquid chromatography. The in vitro disappearance trends were linear for all compounds ( P <0.01). β‐carotene kd were 0.13 and 0.37; lutein, 0.20 and 0.25; total carotene, 0.20 and 0.62 and total xanthophyll, 0.30 and 0.77 h −1 for steers and goats, respectively. The in situ disappearance trends were quadratic ( P <0.01). Dry matter kd were 1.9 and 1.5% h −1 ; cellular content, 2.0 and 2.3; β‐carotene, 2.5 and 1.2; lutein, 2.5 and 1.5; total carotene, 2.2 and 1.0 and total xanthophyll, 2.1 and 1.1% h −1 for steers and goats, respectively. The large disappearance rates of carotenoids observed in the in situ method vs the virtual absence of disappearance in the in vitro method in both species, can be related to the dry matter and cellular content kd. These results suggest that carotenoids disappear probably by joining the cellular content and not by their direct destruction or by attack from the ruminal microorganisms, and the ruminal disappearance is independent of thespecies studied. © 1999 Society of Chemical Industry