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Determination of histamine in fish products by capillary electrophoresis and ion‐pair liquid chromatography with diode‐array detection
Author(s) -
Gardana Claudio,
Pietta Piergiorgio,
Ciappellano Salvatore,
Testolin Giulio
Publication year - 1999
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199901)79:1<91::aid-jsfa185>3.0.co;2-s
Subject(s) - chromatography , capillary electrophoresis , histamine , chemistry , trichloroacetic acid , extraction (chemistry) , high performance liquid chromatography , electrophoresis , fish <actinopterygii> , fishery , biology , endocrinology
A method for histamine determination in fish and fish products by capillary electrophoresis and ion‐pair liquid chromatography has been developed. Histamine was extracted from different fish samples with 10% trichloroacetic acid, and the filtrate was directly applied to capillary electrophoresis apparatus. A preliminary purification through a solid phases extraction cartridge (Sep‐pak C 18 ) was necessary for HPLC analysis. Histamine was detected ‘on‐line’ at 210 nm by both approaches. HPLC procedure allowed accurate (±2.2%) and repeatable (±1.6%) determination of histamine in fish products at appropriate levels (20–100 mg kg −1 ) to meet current regulations. © 1999 Society of Chemical Industry