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Effect of exogenous calmodulin and ATP on the activity of ethylene forming enzyme obtained from tomatoes and green pea pods
Author(s) -
Njoroge Charles K,
Kerbel Eduardo L,
Briskin Donald P
Publication year - 1998
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199802)76:2<215::aid-jsfa937>3.0.co;2-h
Subject(s) - calmodulin , trifluoperazine , biochemistry , adenosine triphosphate , enzyme , ethylene , atp synthase , gramicidin , enzyme assay , chemistry , biology , membrane , catalysis
Ethylene forming enzymes (EFE) from both peas and tomatoes were prepared. EFE from both sources were inhibited by adenosine triphosphate (ATP). Exogeneous calmodulin had no effect on EFE activity either in the presence or absence of ATP. The presence of gramicidin in pea microsomal membrane EFE did not affect EFE activity in the presence of ATP. This suggests that ATP is inhibiting EFE activity through phosphorylation and not through transmembrane proton flux. However, inhibition of EFE activity by ATP was partially reversed by 60 μ M N ‐6(aminohexyl)‐5‐chloro‐1‐naphthalenesulphonamide (W7) and 15 μ M trifluoperazine (TFP) but not by 60 μ M N ‐6(aminohexyl)‐1‐naphthalenesulphonamide (W5), which suggests that the ATP inhibitory affect on EFE is probably controlled by calmodulin. © 1998 SCI.