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Medicagenic acid content in foliage of ten varieties of alfalfa ( Medicago sativa L) cultivated in Mexico
Author(s) -
Pérez Norma,
Peña Silvia,
Vega Salvador,
Noa Mario,
Enríquez Raúl
Publication year - 1997
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199711)75:3<401::aid-jsfa895>3.0.co;2-u
Subject(s) - cultivar , medicago sativa , high performance liquid chromatography , trichoderma viride , bioassay , biology , medicago , horticulture , botany , chemistry , chromatography , biochemistry , genetics , gene
Medicagenic acid (MA) content was studied in four Mexican, one Spanish and five USA cultivars of alfalfa ( Medicago sativa L) using high‐performance liquid chromatography (HPLC) and two bioassays: Trichoderma viride inhibition and ichthyotoxic activity. The MA contents (g kg −1 ), measured through HPLC, for the 10 varieties were: Valenciana=0·165; Inia 76=0·115; Puebla 76=0·097; Synthetic I=0·042; Pierce=0·031; Maxidor=0·027; Condor=0·024; Synthetic II=0·023; Sundor=0·023; NK‐819=0·013. These data confirm the influence of the variety in MA contents. Fungitoxic and ichthyotoxic assays showed that the Spanish cultivar (Valenciana) presented the highest toxicity. The biological activity of the ten varieties was strongly correlated (r=0·97) to medicagenic acid content measured through HPLC. ©1997 SCI