Premium
A simple and rapid method for isolating cassava leaf linamarase suitable for cassava cyanide determination
Author(s) -
Yeoh HockHin,
Bradbury J Howard,
Egan Sylvia V
Publication year - 1997
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199710)75:2<258::aid-jsfa873>3.0.co;2-m
Subject(s) - cyanide , chromatography , enzyme , ammonium sulfate precipitation , chemistry , ammonium , size exclusion chromatography , biochemistry , organic chemistry
A simple method was developed for isolating cassava leaf linamarase. It involved homogenising the leaves in a buffer containing polyvinylpolypyrrolidone, followed by ammonium sulphate precipitation, filtration and partial purification of the enzyme by hydrophobic interaction chromatography. All stages of the enzyme preparation were carried out at room temperature and it was completed within 90 min. A linamarin‐indicator strip for checking the activity of the enzyme preparation was also developed. This isolation technique which has been developed into a simple kit should be suitable for laboratories in developing countries where the enzyme is needed to determine the cyanogenic potential of cassava and its products. © 1997 SCI.