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Pheophytin a Is a Potent Suppressor against Genotoxin‐Induced umu C Gene Expression in Salmonella typhimurium (TA 1535/pSK 1002)
Author(s) -
Okai Yasuji,
HigashiOkai Kiyoka
Publication year - 1997
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199708)74:4<531::aid-jsfa834>3.0.co;2-9
Subject(s) - pheophytin , gene , gene expression , biology , biochemistry , chemistry , microbiology and biotechnology , photosynthesis , photosystem ii
Abstract Previously, a strong antigenotoxic activity was detected in the extract of an edible green alga, Enteromorpha prolifera (Sujiao‐nori in Japanese) (Okai et al 1994 Cancer Lett 87 , 25–32). The present authors studied the active principle corresponding to the antigenotoxic activity in the extract of this alga. A dark‐green spot was detected on a silica gel TLC of the extract of E prolifera which was further purified by a HPLC system and identified to be a derivative of chlo‐rophyll a , pheophytin a , compared with the retention time of HPLC and absorp‐tion spectrum of the standard pheophytin a from Anacystis nidulans . Pheophytin a derived from E prolifera caused a dose‐dependent suppressive effect on umu C gene expression in Salmonella typhimurium (TA 1535/pSK 1002) induced by 2‐aminoanthracene and 3‐amino‐1,4‐dimethyl‐5H‐pyrido[4,3–6]indole in the presence of hepatic metabolising enzymes. Furthermore, the standard pheophytin a from A nidulans also exhibited a similar inhibitory effect on umu C gene expression system at the same concentrations. These results indicate that pheophytin a is a potent suppressor against genotoxin‐induced umu C gene expression in S typhimurium (TA 1535/pSK 1002) probably associated with the carcinogenesis. © 1997 SCI.