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A Simple and Fast PCR Protocol to Detect Listeria monocytogenes from Meat
Author(s) -
Manzano Marisa,
Cocolin Luca,
Ferroni Pierino,
Cantoni Carlo,
Comi Giuseppe
Publication year - 1997
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199705)74:1<25::aid-jsfa759>3.0.co;2-v
Subject(s) - listeria monocytogenes , food science , biology , polymerase chain reaction , detection limit , listeria , contamination , microbiology and biotechnology , chemistry , gene , chromatography , bacteria , genetics , ecology
Primers for the iap gene were used to develop and optimise a PCR technique for detecting Listeria monocytogenes in meat. The use of these primers gave good results in the detection of L monocytogenes in either artificially or naturally contaminated foodstuffs. The lowest sensitivity limit achieved with PCR was 10 1 –10 2 cells ml ‐1 or g ‐1 product, either in pure cultures or in artificially and naturally contaminated meat. The PCR method devised has high sensitivity and specificity. It appears to give good results and is very easy and fast to perform, requiring only 30 h to detect Listeria from meat, whereas, conventional methods require almost 96–120 h. © 1997 SCI.