Premium
Detection of Immunomodulating Activities in an Extract of Japanese Edible Seaweed, Laminaria japonica (Makonbu)
Author(s) -
Okai Yasuji,
Ishizaka Shigeaki,
HigashiOkai Kiyoka,
Yamashita Uki
Publication year - 1996
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199612)72:4<455::aid-jsfa679>3.0.co;2-9
Subject(s) - polysaccharide , polyclonal antibodies , spleen , laminaria , chemistry , fucoidan , biology , food science , biochemistry , microbiology and biotechnology , antibody , algae , immunology , botany
A significant immunomodulating activity was found in the hot‐water‐soluble extract of the most popular edible seaweed in Japan, Laminaria japonica (Makonbu in Japanese) which showed an enhancing activity for DNA synthesis of spleen cells from endotoxin‐nonresponder, C3H/HeJ mice. This activity was divided into polysaccharide and non‐polysaccharide fractions and the former fraction exhibited much higher activity than that of the latter fraction. The polysaccharide fraction caused stimulatory effects on the ingestive activity of mouse phagocytic cells against Staphylococcus aureus and the release of cytokines, interleukin‐1α and tumour necrosis factor α from the same cells. Furthermore, the polysaccharide fraction exhibited enhancing effects on polyclonal antibody (IgM and IgG) production in spleen cells. These immunomodulating activities were associated with polysaccharides themselves, but not contaminating endotoxins in the fraction judged by comparative experiments. The significance of this finding is discussed from the viewpoint of the immunopotentiation by edible seaweeds in host animals.