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Determination of Triacylglycerol Classes and Molecular Species in Seed Oils with High Content of Linoleic and Linolenic Fatty Acids
Author(s) -
Tarandjiiska Roumyana B,
Marekov Ilko N,
NikolovaDamyanova Boryana M,
Amidzhin Bistra S
Publication year - 1996
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199612)72:4<403::aid-jsfa671>3.0.co;2-d
Subject(s) - linseed oil , chemistry , chromatography , thin layer chromatography , linolenic acid , gas chromatography , densitometry , linoleic acid , detection limit , composition (language) , high performance thin layer chromatography , fatty acid , food science , biochemistry , physics , linguistics , philosophy , quantum mechanics
Soya bean and linseed oils were analysed by successive application of silver‐ion and reversed‐phase thin‐layer chromatography and densitometry. Direct quantitative determination of all the 20 possible triacylglycerol (TAG) classes including critical pairs (SD 2 +S 2 T; M 2 T+D 3 ; SDT+MDT; ST 2 +MT 2 ) was performed. TAG molecular species down to 0·1% (32 in soya bean oil and 26 in linseed oil) were identified and quantified. The detection limit was 0·2 μg and the standard deviation between 0·1% and 1·0% for silver ion thin‐layer chromatography (TLC) and between 0.1% and 3.9% for reversed‐phase TLC determinations. The accuracy of the analysis was confirmed by the good agreement between the quantitative results for the fatty acid composition as determined by gas chromatography and as calculated from the densitometric data for the TAG composition.