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Antimutagenicity of Lactic Acid Bacteria from El‐Klila Cheese
Author(s) -
Boubekri Karima,
Ohta Yoshiyuki
Publication year - 1996
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199612)72:4<397::aid-jsfa673>3.0.co;2-e
Subject(s) - lactic acid , bacteria , pediococcus , indole test , chemistry , quinoline , food science , biochemistry , antimutagen , mutagen , tryptophan , amino acid , biology , carcinogen , organic chemistry , genetics
The successful research of lactic acid bacteria (LAB) reflect their potential application as anticarcinogens/antimutagens. We therefore studied in vitro binding assay of mutagenic heterocyclic aromatic amines (HCA) to 11 strains of LAB isolated from the Algerian traditional cheese, El‐Klila. The mutagens were added at a concentration of 100 μg ml −1 to methanolic suspensions of each lyophilised strains (5 mg), and incubated at 37°C for 15 min. HCA retained in samples were quantified by HPLC. The average binding (%) of 3‐amino‐1,4‐dimethyl‐5 H ‐pyrido‐[4,3‐ b ]‐indole (Trp‐P‐1), 3‐amino‐1‐methyl‐5 H ‐pyrido‐[4,3‐ b ]‐indole (Trp‐P‐2) and 3‐amino‐3‐methyl‐imidazo‐[4,5‐ f ]‐quinoline (IQ) were 98·14±0·1, 96·45±0·13 and 34·90±0·5, respectively, and differed significantly ( P< 0·05). Pediococcus sp BK3‐11 had the highest binding (%) activity to Trp‐P‐2 (99·58±0·25) and IQ (69·5±1·6). Lyophilised cytoplasm and cytoplasmic membrane of Pediococcus sp BK3‐11 showed no binding activity, however the cell wall skeletons manifested significant binding (%) to Trp‐P‐2 (98·05±0·21) and IQ (59·56±0·7). The binding system was pH dependent and influenced by metal ions. The studies point out the antimutagenic cell components of lactic acid bacteria.