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Correlation of HPLC and AOAC Methods to Assess the All‐ trans ‐Lutein Content in Marigold Flowers
Author(s) -
DelgadoVargas Francisco,
ParedesLópez Octavio
Publication year - 1996
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199611)72:3<283::aid-jsfa652>3.0.co;2-v
Subject(s) - lutein , saponification , xanthophyll , chemistry , high performance liquid chromatography , chromatography , correlation coefficient , carotenoid , food science , mathematics , statistics
A reversed‐phase C 30 high‐performance liquid chromatography (HPLC) method was used to separate the lutein isomers of marigold flowers. The pigments were extracted from marigold meal by an Association of Official Analytical Chemists (AOAC) modified method. The unsaponified extract showed the presence of several lutein ester isomers and after saponification four major lutein isomers were detected. The relationship between all‐ trans ‐lutein, assessed by HPLC, and total xanthophylls, assessed by AOAC, was determined. A regression equation, with a high correlation coefficient ( r 2 =0·972), which allows for the calculation of all‐ trans ‐lutein from total xanthophylls content is proposed.