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Solid‐Phase Microextraction (SPME) of Pyrazines in Model Reaction Systems
Author(s) -
Ibañez Elena,
Bernhard Richard A
Publication year - 1996
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199609)72:1<91::aid-jsfa627>3.0.co;2-t
Subject(s) - solid phase microextraction , chromatography , chemistry , extraction (chemistry) , sodium hydroxide , gas chromatography , gas chromatography–mass spectrometry , mass spectrometry , organic chemistry
Headspace solid‐phase microextraction (HS‐SPME) combined with gas chromatography (GC) was used for isolating and analysing pyrazines formed in a model system. This system consisted of an aqueous mixture of glucose, glycine and sodium hydroxide. Pyrazine formation was accelerated by heating in a microwave oven. Results were compared with our earlier ones using conventional techniques for isolation of pyrazines. HS‐SPME‐GC showed several advantages over traditional methods. The method is rapid, sensitive, easy to use and reproducible. Headspace and liquid of the model reaction were extracted and the results obtained using headspace sampling showed good agreement with the methylene chloride extraction method. In all cases, coefficients of variation of 5% or lower were obtained when the SPME extraction time was consistent.