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Direct Enzymatic Analysis of Glycerol in Honey: a Simplified Method
Author(s) -
FernándezMuíño Miguel A,
Sancho M Teresa,
Muniategui Soledad,
Huidobro José F,
Sánchez M Pilar,
SimalLozano Jesús
Publication year - 1996
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199606)71:2<141::aid-jsfa547>3.0.co;2-n
Subject(s) - cuvette , glycerol , chromatography , enzyme , chemistry , sensitivity (control systems) , biochemistry , optics , physics , electronic engineering , engineering
The glycerol content of honey has been determined using a Boehringer Mannheim enzymatic method simplified for this purpose. The solution of honey was employed directly, without clarification and without addition of NaOH solution. With these conditions, there were no observed interference effects. It is necessary to use a 1·5 ml micro‐cuvette. The enzymatic determination was measured spectrophotometrically at 365 nm using the enzymes in quadruple the quantities recommended by the supplier. The method combines precision, good recovery, sensitivity, simplicity and low cost.