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Low Trypsin and Chymotrypsin Inhibitor Mutants in Winged Bean (P sophocarpus tetragonolobus (L) DC)
Author(s) -
Kothekar Vijay S,
Harsulkar Abhay M,
Khandelwal Ajay R
Publication year - 1996
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/(sici)1097-0010(199605)71:1<137::aid-jsfa563>3.0.co;2-y
Subject(s) - mutant , chymotrypsin , trypsin , proteases , trypsin inhibitor , biology , biochemistry , gene , microbiology and biotechnology , chemistry , enzyme
Low trypsin inhibitor (LTI) and chymotrypsin inhibitor (LCTI) mutants have been identified and isolated from the M 5 mutant lines of winged bean. The LTI and LCTI lines demonstrated a true breeding feature in the subsequent generations. Three to five isoinhibitor bands of trypsin and chymotrypsin were absent in different mutants, indicating mutations in the respective gene loci. The LTI and LCTI mutants inhibited insect gut proteases as efficiently as their control counterparts. This could be attributed to a specific group of inhibitors present in all the mutant types.