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Comparative genomic hybridization of microdissected samples from different stages in the development of a seminoma and a non‐seminoma
Author(s) -
Looijenga Leendert H. J.,
Rosenberg Carla,
van Gurp Ruud J. H. L. M.,
Geelen Eric,
van EchtenArends Jannie,
de Jong Bauke,
Mostert Marijke,
Wolter Oosterhuis J.
Publication year - 2000
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(200006)191:2<187::aid-path584>3.0.co;2-t
Subject(s) - seminoma , comparative genomic hybridization , biology , germ cell , pathology , cancer research , genetics , genome , medicine , gene , chemotherapy
Human testicular germ cell tumours (TGCTs) of adolescents and adults, both seminomas and non‐seminomas, originate from intratubular germ cell neoplasia (IGCN). Comparative genomic hybridization (CGH) was applied to microdissected samples from different stages of the development of a seminoma and a mixed non‐seminoma, including IGCN of both. The different stages of the seminoma development, namely IGCN, intratubular and invasive seminoma, showed a very similar pattern of chromosomal imbalances, including gains of parts of 7, 8,12,14, and X, and losses of parts of 3, 4, 5, 10, 11, 12q, 16, 18, 22, and Y. A more heterogeneous pattern was found for the non‐seminoma. Some aberrations were present only in IGCN, or in IGCN and in all invasive components (gains of parts of 1q, 17, 19p, 20q, and 22, and losses of parts of 4, 5, 9p, 13, and 18q), while others were present in a less consistent pattern. These are the first reported CGH data from different stages in the development of TGCTs. Although only two cases were studied, the results suggest that particular numerical changes of (parts of) chromosomes are involved in the early development and progression of this cancer. Copyright © 2000 John Wiley & Sons, Ltd.