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20q13 and cyclin D1 in ovarian carcinomas. Analysis by fluorescence in situ hybridization
Author(s) -
Diebold Joachim,
Mösinger Kerstin,
Peiro Gloria,
Pannekamp Uwe,
Kaltz Christine,
Baretton Gustavo B.,
Meier Werner,
Löhrs Udo
Publication year - 2000
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(200004)190:5<564::aid-path569>3.0.co;2-l
Subject(s) - cyclin d1 , fluorescence in situ hybridization , biology , immunohistochemistry , in situ hybridization , serous fluid , pathology , cancer , gene , cell cycle , medicine , messenger rna , genetics , chromosome , immunology , biochemistry
In ovarian carcinomas, alterations of the chromosomal region 20q13 and the cyclin D1 gene have been described. This study has sought to determine their prognostic significance. Fluorescence in situ hybridization (FISH) on dissociated nuclei and paraffin sections with DNA probes for 20q13.2 and cyclin D1, as well as immunohistochemistry (cyclin D1), were applied to formalin‐fixed tissue of 69 invasive ovarian carcinomas, mainly of serous type. On dissociated nuclei 33/47 cases (70%) and on tissue sections 13/66 cases (20%) demonstrated an increase of 20q13.2 copies. The presence of ≥4 copies per nucleus (isolated nuclei) and ≥3 copies per nucleus (sections) was associated with an adverse prognosis (Kaplan–Meier for FIGO stage III after stratification for residual tumour: p =0.0049 and p =0.03, respectively). Thirty‐four out of 47 cases (72%) showed an increase of cyclin D1 copies. Kaplan–Meier analysis for FIGO stage III after stratification for residual tumour>2 cm or ≤2 cm revealed an unfavourable outcome for cases with more than two cyclin D1 copies ( p =0.04). No correlation was seen between FISH and immunohistochemistry. Multivariate analysis identified residual tumour ( p =0.0002), 20q13.2 gain ( p =0.0004) and cyclin D1 gain ( p =0.0343) as independent prognostic factors. It is concluded that gains of chromosomal region 20q13.2 and the cyclin D1 gene are frequent and biologically important events, with prognostic relevance, in advanced ovarian carcinomas. Copyright © 2000 John Wiley & Sons, Ltd.