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Mesothelial regeneration is not dependent on subserosal cells
Author(s) -
Mutsaers Steven E.,
Whitaker Darrel,
Papadimitriou John M.
Publication year - 2000
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(200001)190:1<86::aid-path493>3.0.co;2-g
Subject(s) - mesothelium , mesothelial cell , connective tissue , population , pathology , regeneration (biology) , wound healing , biology , epithelium , chemistry , medicine , andrology , microbiology and biotechnology , immunology , environmental health
It has been proposed that after mesothelial injury, resident cells within the subserosal connective tissue proliferate, differentiate, and migrate to the serosal surface. The aim of this study was to examine the temporal and spatial changes of proliferating cells in a murine model of testicular mesothelial healing and assess the potential of submesothelial cells to reconstitute the damaged mesothelium. Histology and autoradiography were employed to determine the number of cells within the submesothelial connective tissue, as well as the proportion of cells undergoing DNA synthesis on and beneath the injured serosa. Mesothelial cells surrounding the wound demonstrated maximal DNA synthesis 48 h after injury (27.82±5.64% SEM, compared with 0.17±0.16% 3 H‐TdR labelled cells for resting mesothelium), whereas a significant increase in proliferating submesothelial cells was not seen until day 4 post‐injury (7.79±3.31% compared with 0.85±0.64% 3 H‐TdR labelled cells at day 2). Furthermore, this small number of dividing submesothelial cells must include cells other than the proposed mesothelial precursors, indicating a very low proportion of precursor cells in the submesothelial cell population. As large numbers of mesothelial cells were seen at the wound centre by 3–4 days after injury, it is unlikely that submesothelial cells contributed significantly to the repopulation of the injured mesothelium. It is hypothesized that regenerating mesothelium is more likely to originate from the surrounding uninjured mesothelial cell population. Copyright © 2000 John Wiley & Sons, Ltd.