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Morphologically normal, CD30‐negative B‐lymphocytes with chromosome aberrations in classical Hodgkin's disease: the progenitor cell of the malignant clone?
Author(s) -
Jansen Maurice P. H. M.,
Hopman Anton H. N.,
Bot Fredrik J.,
Haesevoets Annick,
StevensKroef Marian J. P. L.,
Arends Jan Willem,
Jox Andrea,
Wolf Jürgen,
Ramaekers Frans C. S.,
Schouten Harry C.
Publication year - 1999
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199912)189:4<527::aid-path488>3.0.co;2-n
Subject(s) - reed–sternberg cell , biology , cd30 , clone (java method) , population , chromosome , pathology , immunocytochemistry , progenitor cell , microbiology and biotechnology , lymphoma , immunology , stem cell , genetics , gene , medicine , endocrinology , environmental health , hodgkin lymphoma
A recent study observed that numerical chromosome abnormalities in Hodgkin's disease (HD) are detected not only in morphologically abnormal Hodgkin/Reed–Sternberg cells, but also in a fraction of morphologically normal cells. However, the phenotypic constitution of these genetically abnormal, morphologically normal cells and their relationship to the malignant Hodgkin/Reed–Sternberg cells could not be established in the earlier cases studied, because of the low frequency of these cells. The present study investigated two cases of classical Hodgkin's disease containing a relatively large population of such apparently normal cells with aberrant chromosome copy numbers. The phenotype and their position within the developmental route of the malignant compartment were examined by a combined in situ hybridization and immunocytochemistry approach. Numerical abnormalities for chromosome 1 in one case and for chromosomes X, Y, and 1 in the other case were observed not only in CD30‐positive Hodgkin/Reed–Sternberg cells, but also in CD30‐negative, morphologically normal cells. It was shown that these genetically aberrant cells expressed the B‐cell antigen CD19, thus confirming their B‐cell nature. These studies indicate a relationship between the genome aberrations in these genetically abnormal, morphologically normal B‐cells and the Hodgkin/Reed–Sternberg cells, suggesting that they are progenitor cells of the malignant cell fraction. Copyright © 1999 John Wiley & Sons, Ltd.