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Isolation and characterization of glioblastoma‐associated homozygously deleted DNA fragments from chromosomal region 9p21 suggests involvement of multiple tumour suppressor genes
Author(s) -
Simons Annet,
Jeuken Judith W. M.,
Eleveld Marc J.,
Boerman Rudolf H.,
Geurts van Kessel Ad
Publication year - 1999
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199911)189:3<402::aid-path437>3.0.co;2-8
Subject(s) - biology , gene , suppressor , tumor suppressor gene , genetics , dna , chromosome , carcinogenesis , cancer research , microbiology and biotechnology
Representational difference analysis (RDA) of a human glioblastoma xenograft resulted in the isolation of five tumour‐associated homozygously deleted DNA fragments, all originating from chromosome 9, region p21. Subsequent analysis of a series of ten glioblastomas using the newly isolated RDA fragments in conjunction with a series of known 9p21 DNA markers revealed homozygous deletions in nine of the ten (90 per cent) tumours. These deletions encompass the p15/p16 complex and two additional putative tumour suppressor loci. The RDA fragments correspond to the latter two loci. Taken together, these results suggest the involvement of multiple tumour suppressor genes from the 9p21 region in glioblastoma tumourigenesis. The novel RDA fragments will be instrumental in the isolation of the relevant genes. Copyright © 1999 John Wiley & Sons, Ltd.