Premium
The common 18‐base pair deletion at codons 418–423 of the E‐cadherin gene in differentiated‐type adenocarcinomas and intramucosal precancerous lesions of the stomach with the features of gastric foveolar epithelium
Author(s) -
Endoh Yasushi,
Tamura Gen,
Watanabe Hidenobu,
Ajioka Yoichi,
Motoyama Teiichi
Publication year - 1999
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199910)189:2<201::aid-path409>3.0.co;2-a
Subject(s) - foveolar cell , exon , biology , pathology , stomach , gene , immunohistochemistry , carcinoma , mutation , mucin , gastric mucosa , microbiology and biotechnology , genetics , medicine , biochemistry
Alterations of the E (epithelial)‐cadherin gene are often involved in undifferentiated‐type carcinomas of the stomach, but not in the differentiated type. Recently, it was recognized that certain differentiated‐type carcinomas possess the features of gastric foveolar epithelium. Such carcinomas are closely linked with undifferentiated‐type carcinomas. Nineteen lesions (ten differentiated‐type carcinomas and nine precancerous lesions) with a predominant gastric foveolar phenotype, as determined not only by mucin histochemistry and immunohistochemistry but also by morphology, were analysed for mutations of the E‐cadherin gene using the nested polymerase chain reaction single‐strand conformation polymorphism method. E‐cadherin gene mutation was detected in 4 of 19 lesions (21 per cent) (three precancerous and one cancerous). Sequencing revealed an 18 bp deletion corresponding to codons 418–423 of exon 9 common to all four lesions. This mutation was previously reported in an advanced carcinoma of undifferentiated type. The results suggest that these tumours progress to undifferentiated‐type carcinomas through loss of E‐cadherin function. Copyright © 1999 John Wiley & Sons, Ltd.