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Interferon‐gamma gene expression during acute graft‐versus‐host disease: relationship to MHC induction and tissue injury
Author(s) -
Parfrey Nollaig A.,
ElSheikh Amr,
Monckton Elizabeth A.,
Cockfield Sandra M.,
Halloran Philip F.,
Linetsky Elina
Publication year - 1999
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199909)189:1<99::aid-path404>3.0.co;2-f
Subject(s) - major histocompatibility complex , interferon gamma , in situ hybridization , biology , immunology , interferon , spleen , gene expression , pathology , immune system , gene , medicine , genetics
The pathogenetic role of interferon‐γ (IFN‐γ) in acute graft‐versus‐host disease (GVHD) was examined in a murine model. IFN‐γ gene expression was evaluated by northern blotting and mRNA in situ hybridization. The temporal and tissue specific patterns of IFN‐γ gene expression were related to the patterns of major histocompatibility complex (MHC) antigen induction and of tissue injury. Markedly increased levels of IFN‐γ transcripts were seen in the spleen during the early lymphoproliferative phase and coincided with widespread MHC induction in non‐lymphoid tissues. Increased IFN‐γ transcripts were also found in the non‐lymphoid target tissues during the phase of subsequent tissue injury. These findings support a role for IFN‐γ in leading to widespread MHC induction during acute GVHD and suggest that IFN‐γ may also contribute to target tissue injury during acute GVHD. Copyright © 1999 John Wiley & Sons, Ltd.