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Expression of the laminin γ2 chain in different histological types of lung carcinoma. A study by immunohistochemistry and in situ hybridization
Author(s) -
Määttä Marko,
Soini Ylermi,
Pääkkö Paavo,
Salo Sirpa,
Tryggvason Karl,
AutioHarmainen Helena
Publication year - 1999
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199908)188:4<361::aid-path363>3.0.co;2-0
Subject(s) - laminin , pathology , immunohistochemistry , carcinoma , biology , stromal cell , in situ hybridization , basement membrane , cell , medicine , gene expression , biochemistry , genetics , gene
Sixty‐four malignant lung tumours and 12 of their regional lymph node metastases were analysed for expression of the laminin γ2 chain by immunohistochemistry and in situ hybridization. Expression of the laminin γ2 chain was strongest in squamous cell carcinomas, followed by adenocarcinomas and large cell carcinomas. Positive cells, except for large cell carcinomas, were located at the epithelial–stromal interface of tumour clusters. An important exception was small cell lung carcinoma, with only a low level of laminin γ2 chain expression. Apart from tumour type, this may reflect the relatively scanty fibrous stroma in these tumours and supports previous observations that small cell lung carcinoma cells, contrary to other types, lack surface expression of α 6 β 4 integrin, the specific laminin‐5 binding receptor. In frozen sections, immunohistochemistry showed linear basement membranes around tumour clusters in squamous cell carcinomas and adenocarcinomas. This shows that carcinoma cells are capable of heavy deposition of the laminin γ2 chain around tumour clusters and suggests that a laminin γ2 chain‐containing substrate may be of significance for the spread and growth of malignant tumours. Copyright © 1999 John Wiley & Sons, Ltd.