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Cyclin D1 expression in astrocytomas is associated with cell proliferation activity and patient prognosis
Author(s) -
Sallinen SatuLeena,
Sallinen Pauli K.,
Kon Juha T.,
Syrjäkoski Kirsi M.,
Nupponen Ni.,
Rantala Immo S.,
Helén Pauli T.,
Helin Heikki J.,
Haapasalo Hannu K.
Publication year - 1999
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199907)188:3<289::aid-path351>3.0.co;2-x
Subject(s) - cyclin d1 , cell growth , cancer research , cyclin , medicine , expression (computer science) , cell cycle , pathology , oncology , biology , cancer , computer science , genetics , programming language
An important positive regulator of the cell cycle, cyclin D1, is often amplified and overexpressed in malignancies. Cyclin D1 aberrations were analysed in grade II–IV astrocytomas by fluorescence in situ hybridization (FISH), mRNA in situ hybridization and immunohistochemistry. Proliferation activity was determined by Ki‐67 MIB‐1 immunolabelling and mitotic counting. High cyclin D1 expression was observed in grade IV astrocytomas (grades II–III versus grade IV; mRNA expression: p < 0·001; immunoexpression: p = 0·013), and correlated with poor patient survival ( p < 0·001, n = 46). Upregulated cyclin D1 expression was also closely associated with poor patient prognosis in grade II–III astrocytomas ( p < 0·001, n = 30). Cyclin D1 gene was not found to be amplified ( n = 7). Cell proliferation activity was significantly increased in tumours exhibiting high cyclin D1 mRNA levels (Ki‐67 MIB‐1 : p < 0·001; mitotic count: p < 0·001) and high cyclin D1 protein expression (Ki‐67 MIB‐1 : p = 0·002; mitotic count: p = 0·012). These results indicate that increased production of cyclin D1 is closely associated with high cell proliferation activity and aggressive behaviour in diffusely infiltrating astrocytomas. Copyright © 1999 John Wiley & Sons, Ltd.