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Expression of CYP2E1 by human monocyte‐derived macrophages
Author(s) -
Hutson Julie L.,
Wickramasinghe Sunitha N.
Publication year - 1999
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199906)188:2<197::aid-path295>3.0.co;2-m
Subject(s) - immunocytochemistry , monocyte , cyp2e1 , messenger rna , circumstantial evidence , macrophage , microbiology and biotechnology , cytotoxicity , biology , reverse transcriptase , gene expression , chemistry , polymerase chain reaction , cytochrome p450 , gene , immunology , enzyme , biochemistry , in vitro , endocrinology , political science , law
Abstract The expression of the ethanol‐metabolizing cytochrome P450 (CYP2E1) in human monocyte‐derived macrophages was studied at the mRNA and protein levels. The presence of mRNA was investigated by reverse transcriptase‐polymerase chain reaction (RT‐PCR) and protein by immunocytochemistry. The data show that CYP2E1 is expressed in human monocyte‐derived macrophages at a level similar to that demonstrated in other extrahepatic tissues. Although there is circumstantial evidence for the presence of CYP2E1 in human macrophages, it has not previously been demonstrated directly. Its presence in macrophages underlines the potential importance of these cells in initiating alcohol‐induced cytotoxicity. Copyright © 1999 John Wiley & Sons, Ltd.