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L‐selectin and its ligands mediate infiltration of mononuclear cells into kidney interstitium after ureteric obstruction
Author(s) -
Shikata Kenichi,
Suzuki Yasuo,
Wada Jun,
Hirata Kyoji,
Matsuda Mitsuhiro,
Kawashima Hiroto,
Suzuki Takashi,
Iizuka Masako,
Makino Hirofumi,
Miyasaka Masayuki
Publication year - 1999
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199905)188:1<93::aid-path305>3.0.co;2-#
Subject(s) - infiltration (hvac) , peritubular capillaries , kidney , peripheral blood mononuclear cell , mononuclear cell infiltration , pathology , chemistry , monoclonal antibody , cellular infiltration , antibody , inflammation , medicine , immunology , endocrinology , biochemistry , physics , in vitro , thermodynamics
It was previously reported that the L‐selectin ligands detected by a rat L‐selectin and human IgG chimeric molecule (rLEC–IgG) are expressed in the distal tubules of the kidney, where no leukocyte traffic is seen under physiological conditions. In the present study, the role of L‐selectin ligands in leukocyte infiltration into the kidney interstitium was investigated using a rat ureteric obstruction model. After ligation of the ureter, ligands for L‐selectin rapidly disappeared from tubular epithelial cells and were relocated to the interstitium and peritubular capillary walls, where infiltration of monocytes and CD8 + T cells subsequently occurred. Mononuclear cell infiltration was significantly inhibited by intravenous injection of a neutralizing monoclonal antibody (MAb) against L‐selectin, indicating the possible involvement of an L‐selectin‐mediated pathway. Interestingly, immunohistochemical studies with a MAb against sulphatide showed that the distribution of sulphatide, known to be one of the candidates of L‐selectin ligand, was almost indistinguishable from the staining pattern of rLEC–IgG in both normal and ureteric obstructed kidneys, suggesting that sulphatide and/or related molecule(s) relocated to the renal interstitium were recognized by leukocyte L‐selectin, leading to interstitial leukocyte infiltration. In line with this notion, intravenous injection of sulphatide markedly inhibited leukocyte infiltration, suggesting that L‐selectin–sulphatide interaction may play a pivotal role in interstitial leukocyte infiltration in the kidney following ureteric obstruction. Copyright © 1999 John Wiley & Sons, Ltd.