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Renal cell carcinomas and pancreatic adenocarcinomas produce nidogen in vitro and in vivo
Author(s) -
Oivula Jan,
Lohi Jouni,
Tani Taneli,
Kangas Lauri,
Kiviluoto Tuula,
Kivilaakso Eero,
Butkowski Ralph,
Virtanen Ismo
Publication year - 1999
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199903)187:4<455::aid-path271>3.0.co;2-c
Subject(s) - biology , pathology , stromal cell , in vivo , cell culture , stroma , adenocarcinoma , immunohistochemistry , basal lamina , cancer research , immunology , cancer , medicine , genetics , microbiology and biotechnology , ultrastructure
The production of nidogen by four renal cell carcinoma (RCC) and three pancreatic adenocarcinoma (PAc) cell lines has been studied in cell culture and in xenografted tumours in nude mice. In RCC cells, immunoreactivity for nidogen was seen only after exposure to monensin to induce cytoplasmic accumulation of secretory proteins. In PAc cells, immunoreaction was also detectable in control cells. Immunoblotting of control and monensin‐exposed cells and immunoprecipitation of culture media of radioactively labelled cells demonstrated the production of nidogen polypeptide of M r ca. 150000 by six of the seven cell lines. Basement membranes (BMs) and stroma of the xenografted tumours derived from these six cell lines demonstrated immunoreactivity for both human and mouse nidogen, as revealed with species‐specific antibodies. The ability of the cells to produce nidogen in vitro and deposit in vivo was positively correlated with high histological grade of the xenografted tumours, although the small number of cell lines studied calls for further studies to confirm this. The distribution of nidogen in human RCC and PAc specimens was also studied by immunohistochemistry. There was strong immunoreactivity for nidogen in tumour stroma, BM of carcinoma cell nests, and endothelial basal lamina, but no conclusions could be drawn regarding histological grade and immunostaining patterns, because stromal production could not be ruled out. The results show that nidogen is produced by human carcinoma cells both in vitro and in vivo . Copyright © 1999 John Wiley & Sons, Ltd.