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Expression patterns of the human papillomavirus type 16 transcription factor E2 in low‐ and high‐grade cervical intraepithelial neoplasia
Author(s) -
Maitland Norman J.,
Conway Sarah,
Wilkinson Nafisa S.,
Ramsdale Jane,
Morris Jo R.,
Sanders Cyril M.,
Burns Julie E.,
Stern Peter L.,
Wells Michael
Publication year - 1998
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(1998110)186:3<275::aid-path159>3.0.co;2-e
Subject(s) - cervical intraepithelial neoplasia , polyclonal antibodies , intraepithelial neoplasia , koilocyte , pathology , epithelium , human papillomavirus , biology , hpv infection , antibody , cervical cancer , cancer research , medicine , immunology , cancer , genetics , prostate
Specific antibodies against the C‐terminus of E2, produced by affinity purification of polyclonal antisera, have been used to identify the cellular populations which express the HPV 16 E2 transcription factor, in a series of formalin‐fixed, paraffin‐embedded cervical tissues. Cases were selected for both the presence of HPV 16 DNA (confirmed by multiple gene‐specific PCR detections) and the presence of multiple grades of cervical intraepithelial neoplasia (CIN). The data indicate that E2 expression is highest in CIN I and in koilocytic lesions. Lower expression was observed in CIN II and little in CIN III lesions. In contrast, there was some restoration of E2 expression in invasive carcinomas, although the intracellular distribution was much more diffuse. The location of E2 expression to the superficial layers of the cervical epithelium, as well as the occurrence of some basal expression in CIN I, suggests that antibodies against HPV 16 E2 could be a useful adjunct to standard histological techniques for the detection of ‘at‐risk’ patients as part of a cervical screening programme.Copyright © 1998 John Wiley & Sons, Ltd.